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Laboratory Equipment Supplier Emergency Braking Automatic Rotary Microtome. Trimming is normally done at a thickness of 20-30. Sectioning The quality microtome blade must be sharp and free of notches, and regularly replaced. This can make up for the misses in the pre-treatment process. Mounting Putting the sections in the waterbath to flatten and smoothen. If the sections are still wrinkling, add alcohol solution to water as it lowers the surface tension and removes wrinkles. Alternate thick and thin sections It may be taken for granted that if the thickness of the section is set on the machine, the slices will come out at the desired thickness.